Which cell lines are of human origin?
Which cell lines are of human origin?
The first human cell line, HeLa, was established in 1951 from a cervical cancer (Scherer et al., 1953).
What are human cell lines?
Human cell lines are immortalized cells propagated in vitro from primary explants of human tissue or body fluid. The use of human cell cultures as a model for more complex biological systems is an integral part of molecular biology, and biomedical research.
What are the 3 types of macrophages?
Macrophages can be classified on basis of the fundamental function and activation. According to this grouping there are classically-activated (M1) macrophages, wound-healing macrophages (also known as alternatively-activated (M2) macrophages), and regulatory macrophages (Mregs).
What is monocytic cell line?
The THP-1 cell line is a human monocytic leukaemia cell line which was established in 1980 by Tsuchiya et al. (1980). It was derived from the blood of a patient with acute monocytic leukaemia. THP-1 cells resemble primary monocytes and macrophages in morphology and differentiation properties.
Are there other cell lines like HeLa?
HeLa cells – a widely used human cell line isolated from cervical cancer patient Henrietta Lacks. HEK 293 cells – derived from human fetal cells. Jurkat cells – a human T lymphocyte cell line isolated from a case of leukemia. Ptk2 cells – derived from male long-nosed potoroo epithelial kidney cells.
Why are Henrietta Lacks cells immortal?
Lacks’ cancer was a uniquely aggressive case, and her biopsy sample doubled in volume every 20 to 24 hours where other cultures would normally die out. If they were fed the right mixture of nutrients to allow them to grow, the cells were effectively immortal.
Are all immortalized cell lines cancerous?
There are various immortal cell lines. Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the in vitro equivalent of cancerous cells.
What are normal cell lines?
C28/I2 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.
What are M1 and M2 macrophages?
M1 macrophages, also called classically activated, respond to stimuli such as LPS, IFN-γ, and are important producers of pro-inflammatory cytokines. M2 macrophages, also called alternatively active respond to stimuli such as IL-4 or IL-13, are producer of anti-inflammatory cytokines.
What cell types are macrophages?
macrophage, type of white blood cell that helps eliminate foreign substances by engulfing foreign materials and initiating an immune response. Macrophages are constituents of the reticuloendothelial system (or mononuclear phagocyte system) and occur in almost all tissues of the body.
Why are U937 cells used?
U937 cells were isolated from a histiocytic lymphoma of a 37-year-old male patient in 1974. Because they are one of just a few cell lines available that express many monocytic characteristics, they are often used to study the behavior and differentiation of monocytes.