How do you identify Lactobacillus acidophilus?
How do you identify Lactobacillus acidophilus?
The bacteria were successfully isolated from a commercial yoghurt drink and identified as L. acidophilus using gram staining, scanning electron microscope, motility, catalase and carbohydrate fermentation tests. The identity of L. acidophilus was further confirmed using Biolog rapid identification system.
What is Lactobacillus species by PCR?
Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene sequences.
How do you identify Lactobacillus species?
Conclusion. Lactobacillus isolates and several other strains of bacteria co-isolated on MRS medium from gastrointestinal ecosystem and fermented food products could be identified using DNA fingerprints generated by restriction endonucleases.
What is a species specific primer?
Species-specific primer pairs that produce a single band of known and different product size have been developed for each member of the clade with the exception of S. pastorianus, which is a polyphyletic allopolyploid hybrid only found in lager breweries, and for which signature sequences could not be reliably created.
What are the characteristics of Lactobacillus acidophilus?
L. acidophilus is a homofermentative, microaerophilic species, fermenting sugars into lactic acid, and grows readily at rather low pH values (below pH 5.0) and has an optimum growth temperature of around 37 °C (99 °F). L. acidophilus is found in the human and animal gastrointestinal tract and mouth.
What color is Lactobacillus acidophilus?
Bacteria Collection: Lactobacillus acidophilus Additional Information
| Fermentation Tests Text: | Maltose : -,Raffinose : – |
|---|---|
| Reduction Text: | Nitrous acid reduction : – |
| Temperature For Growth Text: | at 37°C : Yes |
| Colony Appearance Text: | rough : Yes,shiny : Yes |
| Colony Color Text: | grey : Yes |
What is Lactobacillus species normal range?
Lactic acid is produced by Lactobacillus which maintains the vaginal pH between 3.8 and 4.2. This environment favors the growth of Lactobacillus and inhibits the growth of pathogenic bacteria.
What is vaginalis by RT PCR?
vaginalis infection by PCR is a sensitive and specific method that could be incorporated into a joint strategy for the screening of multiple STDs by using molecular amplification methods. Trichomonas vaginalis infection is the most prevalent nonviral sexually transmitted disease in the world (18).
How do you design a specific primer?
Taking into consideration the information above, primers should generally have the following properties:
- Length of 18-24 bases.
- 40-60% G/C content.
- Start and end with 1-2 G/C pairs.
- Melting temperature (Tm) of 50-60°C.
- Primer pairs should have a Tm within 5°C of each other.
- Primer pairs should not have complementary regions.
What are primers and how are they used in PCR?
In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified, resulting in millions and millions of copies in a very short timeframe. Primers are also used in DNA sequencing and other experimental processes.
What is Lactobacillus acidophilushomology?
Lactobacilli are one of the frequently used commercial probiotic groups within LAB and are considered to play a beneficial role in the human and animal intestinal tract [6–9]. The Lactobacillus acidophilushomology group is a clade of homologous Lactobacillusspecies categorized using molecular-based phenotypic and genotypic techniques [10,11].
What is the PCR primer for Lactobacillus species?
This study aimed to develop a novel multiplex polymerase chain reaction (PCR) primer set for the identification of seven probiotic Lactobacillus species such as Lactobacillus acidophilus, Lactobacillus delbrueckii, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus re …
How difficult is it to identify members of the Lactobacillus acidophilus group?
These results indicated that the members of the Lactobacillus acidophilusgroup were difficult to identify using only 16S rRNA sequences, thus requiring more sensitive detection methods. Table 2 16S rRNA sequence identity percentage of six species of the Lactobacillus acidophilusgroup. Strains L. helveticus L. gallinarum L. acidophilus L. crispatus
How many species of Lactobacillus acidophilus are identified using multiplex PCR?
Genome-based species-specific primers for rapid identification of six species of Lactobacillus acidophilusgroup using multiplex PCR
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