# How much volume does a 384-well plate have?

## How much volume does a 384-well plate have?

The Standard Thermo Scientific 384-well plate is compatible with the majority of 384 block PCR and sequencing instruments. It has a maximum well volume of 40 µL and a working volume of 25 µL. This 384-well plate is compatible with all major 384 block thermal cyclers, and it has a cut corner at the A24 location.

## How many cells are in a 384-well plate?

13. 500 cells / µL (15,000 cells/well/30 µL), or 500,000 cells/mL, 7 mL of cells is sufficient for half of a 384- well plate, so roughly 3.5 million cells are required to plate at a density of 15,000 cells/well in half of a 384-well plate.

**How many mL can a 6 well plate hold?**

Growth Area of Tissue Culture Plates and Dishes

Growth Area (cm2) | Maximum Volume (ml) | |
---|---|---|

150 mm | 148 | |

245 mm (square) | 500 | |

Dishes | ||

6 well | 9.5 | 16.8 |

**How much media can 24 well plate?**

CulturPlate Microplates

Well format | 24-well | 1536-well |
---|---|---|

Recommended working volume | 0.5-2.39 mL | 4 µL-12 µL |

Height (mm) | 18.70 | 14.35 |

Length (mm) | 127.80 | 127.76 |

Width (mm) | 85.60 | 85.48 |

### How much does a 384 well plate weigh?

96 well plates have a volume of 1,200 µL per well; 384 well plates have a volume of 250 µL per well; and 1536 well plates have a volume of 50 µL per well. Total weight including inserts is 289 g, 23.5 g and 768 g respectively for 96, 384 and 1536-well…

### How many microliters are in a 384 well plate?

384 well flat bottom, non-treated microtiter plate with an optimal working volume of 15-145 μL; maximum well capacity of 152 μL.

**How do I calculate my cell number for a well?**

For a 12 well plate, add 3 extra wells to account for any pipetting error/interfering bubbles.

- The equation to solve for would be:
- ‘HAVE’ vs ‘WANT’
- C1V1 = C2V2 2,590,000 cells/mL * (X mL) = 500,000 cells/mL * (15 mL)
- X mL = (500,000 cells/mL * (15 mL)) / 2,590,000 cells/mL.

**How much media do I need for 6 well plates?**

I usually add 1-2 mL of media in each well in the 6-well plate.

## How many wells are in PCR?

384-well

Many PCR Thermal Cyclers/ Thermocyclers on the market today offer a 384-well configuration. Most likely, 384-Well PCR Thermal Cyclers/ Thermocyclers will also be able to accommodate 96-well blocks as well as 48-well or even dual 48-well blocks.

## What are microtiter plates used for?

Microtiterplates are convenient, high-throughput tools for organizing tissue culture, PCR tests (such as HIV screening), or immunological assays such as ELISA, RIA, and FIA.. Other applications for PCR plates include analysis of enzyme reactions and compound identification by colorimetric or spectroscopic analysis.

**How do I calculate water volume in a well?**

First, subtract the depth to water from the overall depth of the well. For example, if our well was ten feet deep and it was a foot to water, we’d have nine feet of water. Next, use the following equation: pi (or 3.15 if you’re not using a calculator) times the square of your radius times the height of your well.

**How many gallons are in a well?**

The typical 6-inch diameter well will hold approximately 1.5 gallons of water per foot of casing. The height of the water above the pump when it is not operating, multiplied by the gallons of water per foot of casing approximates the amount of available storage within the well casing.