What is pGreen plasmid?
What is pGreen plasmid?
The pGreen plasmids are vectors for plant transformation. They were first described in 2000 as components of a novel T-DNA binary system. The supporting web page provides supplementary information and ongoing support to researchers to request their plasmid resources.
What is Agrobacterium-mediated transformation?
Agrobacterium-mediated transformation (AMT) heavily relies on the capability of bacterial pathogen Agrobacterium tumefaciens in transferring foreign genes into a wide variety of host plants. Currently, AMT is the most commonly used method for generating transgenic plants.
What are applications of Agrobacterium-mediated plant transformation?
Applications of transgenic plants. Agrobacterium-mediated transformation has been used for genetic modification of plants for production of various useful proteins, such as recombinant antibodies (plantibodies) and edible vaccines (plantigens) (Daniell et al., 2001).
Why is Agrobacterium-mediated?
Agrobacterium-mediated gene transfer in plants is a highly efficient transformation process which is governed by various factors including genotype of the host plant, explant, vector, plasmid, bacterial strain, composition of culture medium, tissue damage, and temperature of co-cultivation.
What is used to make the green in pGREEN plasmids?
The pGREEN plasmid contains a mutant version of GFP linked to another gene called beta-galactosidase. This combination of genes was chosen because the protein produced from this combination turns bacteria yellow-green, even in normal light. If you expose the colonies to a UV light, they also fluoresce.
What is PBIN19?
Explanation: PBIN19 is a disarmed cloning vector which is a binary vector. The left and right borders of this vector flank copy of lacZ’ gene.
How does Agrobacterium mediated gene transfer work?
The bacteria enters the plant through cuts or wounds present in its root or stem. The bacteria then inserts its DNA and stimulates the plant to grow swollen galls. Agrobacterium tumefaciens is capable of interkingdom DNA transfer, thus making it a potential vector in the production of transgenic plants.
What is the main advantage of Agrobacterium mediated gene transfer?
The advantages of Agrobacterium-mediated transformation include the transfer of pieces of DNA with defined ends and minimal rearrangement, the transfer of relatively large segments of DNA, the integration of small numbers of copies of genes into plant chromosomes and the high quality and fertility of transgenic plants.
Why does transforming the pGREEN plasmid into the bacteria cause the bacteria to turn green?
When inserted into a plasmid and used for the transformation procedure, the transformed bacteria will express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow green under ultraviolet light.
What causes the green fluorescence of colonies?
Note: The pGREEN plasmid contains a mutant version of GFP linked to another gene called beta-galactosidase. This combination of genes was chosen because the protein produced from this combination turns bacteria yellow-green, even in normal light. If you expose the colonies to a UV light, they also fluoresce.
What is PBIN19 vector?
Plasmid: pBin19 (ATCC staff) A vector for efficient transformation of higher plants in a binary plasmid system with pAL440. (personal communication) Medium is 1065 LB medium. NCBI gi: 520486 NCBI gi: 529322 Hosts: Agrobacterium tumefaciens, E. coli HB101, higher plants, E. coli.
What is PBIN 19 Mcq?
What is Pbin19? Explanation: PBIN19 is a disarmed cloning vector which is a binary vector. The left and right borders of this vector flank copy of lacZ’ gene.