What is the Cre lox system used for?
What is the Cre lox system used for?
The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.
Is Cre lox system reversible?
Since the lox sites remain unchanged, this process is reversible and the gene can “flip” back and forth between both orientations indefinitely. Translocation. If lox sites reside on separate pieces of DNA, recombination will result in a reversible translocation event.
What is the Cre method?
The CRE Methodology™ is iterative; subsequent experimental plans will be based on the outcome of the previous experiments. Each improvement builds upon the success of the previous ones. Each time your conversion rate is increased, it becomes increasingly difficult for competitors to compete.
How does Cre ERT2 work?
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with …
How does CRE mediated recombination work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
What does Cre stand for?
carbapenem-resistant enterobacteriaceae
CRE, which stands for carbapenem-resistant enterobacteriaceae, are strains of bacteria that are resistant to carbapenem, a class of antibiotics typically used as a last resort for treating severe infections when other antibiotics have failed.
What does Cre dependent mean?
Cre-dependent gene expression – placing a stop codon with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene expression proceeds.
How does tamoxifen induce Cre?
It utilizes a mutated estrogen receptor (ER) fused to Cre as a transgene (Cre-ER), which only becomes activated and then translocates into the nucleus upon binding of the active tamoxifen (TAM) metabolite 4-hydroxytamoxifen (4-OHT), which mutates the ER.
How long does it take for Cre recombinase to work?
By lipofection, it would take about one day for the Cre to be expressed and then it should be immediately active.